Mono may refer to:

• Infectious mononucleosis, also referred to as "glandular fever", "kissing disease" or "mono"
• Monodactylus sebae, an animal species

• Mono (Japanese band), a Japanese post-rock band
• Mono (UK band), a UK pop duo
• Mono, a 2002 album recorded by Paul Westerberg (as "Grandpaboy") and included with his album Stereo

• mono (Fanzine), Bradford alternative rock music fanzine

• Mono, California, former name of Mono Mills, California
• Mono County, California
• Mono Department, Benin
• Mono Lake, California
• Mono, Ontario, Canada
• Mono River, Togo
• Mono Island, Solomon Islands

• Mono tribe, a tribe of Native Americans in California
  • Mono language (Native American), their language
• Mono, an important character in the video game Shadow of the Colossus

• Mono (software), an open source implementation of Microsoft's Common Language Infrastructure
• Monochrome BBS, a text-based bulletin board system originally based at City University, London
• Everson Mono, also known as Everson Mono Unicode, is an evenly spaced Unicode font (typeface)
• m0n0wall is an embedded firewall distribution of FreeBSD
• Mono (game), a shoot 'em up video game reminiscent of both Asteroids and Robotron: 2084

• Monophonic
• Monochrome Graffiti, a type of graffiti style
• Mono, a type of rock climbing hand hold in which only one finger can be used
• Mono-, a Numerical prefix
• Monomorphism, a kind of morphism in universal algebra or category theory
• Mono, an international currency created by Sennacia banko

• Poly
• Solo (music)
• Stereo